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doi: 10.2174/1389200222666210202110129 186

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Membranes were blocked with TBST buffer containing 5% non-fat milk for 1 h and incubated with the primary antibody in primary antibody diluent (10X, Beyotime) overnight at 4 °C: MFN2 (ABclonal A19678), DRP1 (Abcam ab184248), HSP60 (Abcam ab190828), VDAC1 (Merck MABN504), TFAM (Abcam ab131607), OXPHOS (Abcam ab110413), GAPDH (Proteintech 81640-5-RR), PLOG (Abcam ab128899), MRPS30 (Proteintech 18441-1-AP), MRPL28 (Proteintech 68467-1-Ig), RPS27 (Proteintech 15355-1-AP), LARS2 (Affinity DF13122), TOMM40 (ThermoFisher PA5-110507), TOMM20 (Cell Signaling Technology D8T4N) and β-actin (ABclonal AC026)