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2.11 Separation and extraction of nuclear and cytoplasmic proteins In this experiment, nuclear and cytoplasmic fractionation was performed using the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, P0027) following the manufacturer’s instructions

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Some preparations and some fragments appeared to influence adipose tissue without producing the growth response, which suggested to several groups that the protein might be functionally modular: a growth-promoting activity requiring the intact fold and its receptor-dimerizing surfaces, and a metabolic activity carried by a smaller and more portable piece