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The mapping, as well as the calculation of the percentages of methylation of cytosines in CG context were performed with the Bismark software ( CCL3 functional annotations, such as transcription start site, single nucleotide polymorphisms and predicted transcription factor binding sites were identified with the UCSC Genome Browser ( Statistical analysis Statistical analyses were performed using GraphPad Prism software (version 8.0.2) (GraphPad Software Inc., Irvine, CA)

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By inhibiting NNMT, 5-Amino-1MQ increases intracellular NAD⁺ levels and enhances metabolic activity: NNMT Inhibition: Reduces methylation-driven energy conservation pathways, shifting the body toward increased energy expenditure¹⁻³