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The PCR reaction was carried out in a master cycler (Applied Biosystem) with following reaction cycle: 3 min of initial denaturation (94 °C) followed by 35 cycles of denaturation for 1 min (94 °C), respective annealing temperature for 1 min, extension for 1 min (72 °C) and a final extension step for 7 min (72 °C)

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Graves’ disease: Graves’ disease is another autoimmune condition of the thyroid characterized by the production of TSIs that acts on TSH receptors found on the plasma membrane of thyroid follicular cells, triggering an excessive response by inducing a surge in secretion that eventually causes hyperthyroidism (Bartalena et al