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Thus, the results are highly encouraging, and their only limitation is the fact that it is unknown what percentage of women were diagnosed with infertility [102]

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Lyse Sf9 cells expressing your protein of interest in ~20 ml of GST binding buffer (150 mM NaCl, 1 mM EDTA, 0.05% Triton-X-100, 1 mM DTT in PBS1X for a final concentration of NaCl of 250 mM, and protease inhibitor cocktail (Roche)

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