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Size:330 caps

A mixture including 98 µL of bacterial cultures and 2 µL of 2′,7′-dichlorodihydrofluorescein diacetate (100 µL, final concentration 20 µM) was incubated in microplates at 37°C for 1 h in the dark and observed using 35-mm laser scanning confocal microscopy at the wavelength of 488 nm (OLYMPUS, Japan)