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aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF+aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 ⁇ 10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 ⁇ 10 M Dihexa (Harding lab) in aCSF, 10 ⁇ 12 M Dihexa in aCSF or 50 ng/ml HGF+10 ⁇ 12 M Dihexa in aCSF for 30 minutes at 37° C

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