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The condensation of 4-hydroxycoumarin (1) with pyrene-1-carboxaldehyde (2a ) gave dimeric coumarin derivative PBHC ( 3a) bearing an aryl substituent in the central methylene linker (Scheme 1), while the reaction of 4-hydroxycoumarin (1) with trans-4-methoxycinnamaldehyde (2b) gives MPBHC (3b) (Scheme 2).The chemical identities and structures of PBHC and MPBHC were confirmed by FT-IR, ¹H, ¹³C NMR spectroscopy and high-resolution mass spectrometry (HRMS) (supplementary data)

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Despite the marked decline in PINK1 and parkin, accumulation of LC3-II alongside p62 elevation clearly suggests the notion of impaired autophagic degradation rather than autophagy activation