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The quantitation of peptides using isotopic labeling is performed based on the intensity of peptides measured in MS1 spectra, while that of the isobaric tagging approach is carried out based on the intensity of tags cleaved from labeled peptides in MS2 spectra with signature masses (e.g., 126, 127, 128, 129, 130, and 131 m/z)

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The results showed that the AUC of ZNF83 was 0.934 in the PTLDS dataset and 0.765 in the RA dataset ( Figures 3H, I ), suggesting that ZNF83 has a certain diagnostic discriminative ability in both diseases, although its performance in RA is relatively weaker, and its stability still requires further validation in independent cohorts