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Then, 100 ng INPUT RNA or ENRICH RNA with beads from 100 ug pre-enriched total RNA was fragmentated in Frag/Prime Buffer at 85°C for 6 min and then reverse transcribed in the mixture with 7 μl of First Strand Buffer and 2 μl of First Strand Synthesis Enzyme Mix, following incubation at 25 °C for 10 min, 42 °C for 15 min, 70 °C for 15 min

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[DOI] [PMC free article] [PubMed] [Google Scholar] 169.Park HY, et al