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FIGURE 2 FIGURE 3 FIGURE 4 FIGURE 5 FIGURE 6 FIGURE 7 O -demethylated metabolite i.e., 9- O -demethylmitragynine were detected as the prevalent metabolites of mitragynine, in accord with O -demethylmitragynine was the most abundant metabolite produced by CYP2C19, 2D6, and 3A4, while 9- O -demethyl-16-carboxymitragynine was the least prevalent metabolite hydrolyzed by 2C19