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Deletion of MET10 , one of the two genes encoding the heterotetrameric enzyme sulfite reductase, as well as MET17 , which encodes homocysteine synthase - results in a yeast strain that cannot produce sulfide from inorganic sulfate via sulfate assimilation, and even when provided sulfide exogenously, cannot incorporate the sulfide into organic sulfur metabolites
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Generally, hydrophobic residues form essential contacts during protein-protein interactions, whereas acidic residues mediate long-range interactions with positively charged residues 50-53
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