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The ESI source conditions were set as follows: Ion Source Gas1 (Gas1) as 60, Ion Source Gas2 (Gas2) as 60, curtain gas (CUR) as 30, source temperature: 600°C, IonSpray Voltage Floating (ISVF) ± 5500 V

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performed whole-exome sequencing on paired biopsy samples from baseline and relapsing lesions of four patients with metastatic melanoma