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The samples were alkylated for 45 min at room temperature, mixed with 15 μL non-reducing Laemmli buffer (4% SDS, 120 mM Tris/HCl p H 6.8, 20% glycerol, 0.02% bromophenol blue) and subjected to 12% SDS PAGE

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Separation, purification, analytical control, handling, and final packaging all affect whether a peptide raw material can be supplied consistently from batch to batch