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20 μl reactions contained 1 μg DNA template, 1x reaction buffer, 2.5 mM of each rNTP (Roche), 40 U RNase inhibitor (Thermo Fisher Scientific), and 6 U Large Fragment of DNA Polymerase I (Thermo Fisher Scientific)
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Type:Vial (4ml)
Mechanistically, this compound can inhibit the NF-κB pathway and NLRP3 inflammasome activation, reducing inflammatory responses, restoring gut barrier function by enhancing goblet cells and tight junction proteins (ZO-1 and occludin) and modulating intestinal microbiota diversity [566]
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