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To validate the optical control of Npu DnaE for the activation of protein assembly, Npu DnaE was placed within mCherry so that a cysteine was present at both the N- and C-terminus of the intein, as is required for the second step of splicing, transesterification (Figure 27A)
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This comparison examines how these structural differences translate into distinct pharmacological profiles relevant to researchers working with IGF-1 signaling
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