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Patient declaration, consent, and signatures Include explicit acknowledgement checkboxes: the patient has read and understood the information, disclosed all medical history, understands the risks, has been informed of alternatives, had the opportunity to ask questions, consents to before-and-after photographs, understands results are not guaranteed, and gives informed consent to proceed

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At mid-log the ODs of the bacteria were normalized to OD1, washed twice in sterile PBS and resuspended in sterile PBS