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On the second day, the slides were washed three times for 10 min with TBS including 0.1% Tween-20 and incubated with secondary antibodies: 2 mg/ml Abberior STAR fluorescence 635p, Goat anti Mouse (Abberior) and 2 mg/ml Abberior STAR fluorescence 580, Goat anti Rabbit (Abberior) for 1 h
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