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Purified sHASEGP eluted as a single peak from 24 to 26 minutes from a Pharmacia 5RPC styrene divinylbenzene column with a gradient between 0.1% TFA/H 2 O and 0.1 % TFA/90% acetonitrile/10% H 2 O and resolved as a single broad 61 kDa band by SDS electrophoresis that reduced to a sharp 51kDa band upon treatment with PNGASE-F