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Key Research Areas for 5-Amino-1MQ NNMT enzyme inhibition assays — IC50 characterisation, selectivity profiling, and 1-MNA production quantification NAD+ salvage pathway studies — nicotinamide flux, NMN and NAD+ pool modulation in vitro SAM/SAH methyl-donor pathway research — epigenetic methylation capacity and histone modification studies Adipocyte biology — lipogenesis suppression, adipocyte size, white adipose mass, and lipid storage pathway studies SIRT1 and SIRT3 activation and downstream pathway research — insulin sensitivity, gluconeogenesis, mitochondrial function Skeletal muscle function and sarcopenia pathway research — muscle strength, endurance, and mitophagy-related studies in aged models Gut microbiome modulation research in diet-induced obesity models Cancer cell biology — NNMT-overexpressing cell line studies, anti-proliferative pathway investigations Cardiovascular metabolic pathway research — homocysteine, SIRT activity, NF-κB and STAT3 inflammatory signalling Comparative NNMT inhibitor studies — 5-Amino-1MQ vs