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Microinjection was performed using the following injection mix: KCl (25 mmol L −1 ), Hepes pH 7.4 (7.5 mmol L −1 ), tracrRNA (200 ng μL −1 ), dpy-10 crRNA (150 ng μL −1 ), dpy-10 ssODN (13.75 ng μL −1 ), adh-5 sgRNA (300 ng μL −1 ), adh-5 ssODN (100 ng μL −1 ), Cas9 (416 ng μL −1 , NEB, USA) 59
energie et endurance
Strength:5mg+5mg
An effective method for extracting Moringa leaves with an organic solvent is the Soxhlet device
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