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Following conditioning, the mice were sacrificed, and tissues were collected (gastrocnemius skeletal muscle, subcutaneous adipose, and liver) and were either snap-frozen and stored at 80 °C for ATP quantification or kept on ice for mitochondrial respirometry and GSH/GSSG quantification

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Materials and Methods Participants A total of 276 healthy participants (110 males and 166 females, age range 20–70 years, mean ± standard deviation = 43.63 ± 11.94 years) were recruited for this study (Table 1)

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