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23 In another study on mice (two groups consisted of non-treated control males and antibiotic treated males), microbiota depletion from weaning onwards by means of chronic treatment with antibiotics [antibiotic cocktail consisted of Ampicillin (1 mg/ml), Vancomycin (5 mg/ml), Neomycin (10 mg/ml), Metronidazole (10 mg/ml) and supplemented with Amphotericin-B (0.1 mg/ml)] impacts on anxiety, cognitive behavior and key neuromodulators (tryptophan, monoamines and neuropeptides) of gut-brain-axis suggesting that dysregulation of this axis may contribute to the pathogenesis of disorders associated with altered anxiety and cognition