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Dead space , the volume trapped in the needle hub after the plunger bottoms out, is minimized in fixed-needle insulin syringes but is real in detachable-needle designs, and it becomes proportionally significant at very small volumes
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[16] [17] In NNMT-inhibition experiments, AMPK phosphorylation should be treated as a downstream cellular response rather than a direct NNMT target-engagement marker
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