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When the experiment was run in serum-containing medium the distinction partly collapses anyway, because free GHK will acquire copper from the medium, but that only means the effective species was never under the experimenter's control

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The final incubation volume of 500 µL was made of 385 µL mixed buffer, 12.5 µL of human liver S9, 50 µL of NADPH, 50 µL of UDPGA and 2.5 µL substrate