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Numerical values for the parameters characterizing monotonic concentration-response curves were obtained from fits of the data with a standard sigmoid saturation curve of the form: where X is the logarithm of the free Zn 2+ concentration that produces the effect Y, A 1 is the bottom asymptote, A 2 the top asymptote or maximum effect (E max ), K Zn the free Zn 2+ concentration producing 50% of the E max (= apparent dissociation constant for Zn 2+ ), and h the Hill slope