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For non-voltage gated potassium channels such as GIRK, this might be expected to reduce its relative repolarization influence compared to voltage-gated KCNQ potassium channels, due to a combination of reduced K + driving force, and fixed intrinsic outward conductance for GIRK channels versus non-linear voltage-dependent increases in conductance for KCNQ channels

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The resulting supernatants were aliquoted and used for Enzyme-Linked Immunosorbent Assay (ELISA) analyses