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Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins | Proteintech Group

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Furthermore, by using BSO − a chemical inhibitor of glutamate cysteine ligase (GCL), an enzyme responsible for glutathione synthesis − we depleted glutathione levels in A549 cells and observed significant increase in sensitivity to cisplatin, confirming the importance of glutathione in determining cisplatin resistance