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In brief, EV fractions containing 0.32 ΞΌg of protein or purified Rac1 protein were treated with 4 ΞΌM trypsin (MP Biomedicals) in PBS for 30 min at 37 Β°C
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[158] detected various PhACs, including analgesics, anti-inflammatories, and psychiatric drugs, in fish from the Ebro River Delta (Spain), with notable accumulation in fish liver (up to 0.166 mg/kg), plasma (up to 63 ng/mL), and muscle (up to 0.031 mg/kg)
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