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METHOD DETAILS Cell sorting and Flow cytometry To stain extracellular surface molecules, the cells were incubated in FACS buffer (PBS with 1% FCS and 5mM EDTA pH 8.0) together with specific antibodies for at least 30 minutes at 4°C protected from light
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This single-stranded RNA virus with a 29.9 kb genome encodes 14 open reading frames (ORFs) and initiates a plethora of virus–host protein–protein interactions in the human body
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