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Key Words: Erythropoietin, Hypoxia-inducible factor-1, Methotrexate, Vascular endothelial growth – factor, Vitamin B12 Introduction Methotrexate (MTX) is a folate antimetabolite and cytotoxic chemotherapeutic agent used in the treatment of different malignancies such as head and neck tumors, osteosarcoma, and acute lymphoblastic leukemia (1)
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