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Heterogeneous refinement and subsequent 3D classification were able to enrich for DDX18 density, leading to a 2.35 Å reconstruction from non-uniform refinement of 131,583 particles following reference-based motion correction, global contrast transfer function refinement, and local contrast transfer function refinement

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the system configured to perform a method according to the eighth aspect of the disclosure wherein the detecting is performed according to any one of the methods according to the sixth aspect of the instant disclosure and/or using any of the related databases the system comprises exome sequences databases and/or reagents to detect exome sequences in an individual of the biological organism, in combination with reagents to perform proteomic analysis of the biological organism