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When cardiolipin is reduced or lost, cytochrome c is free to move to the cytoplasmic membrane and initiate apoptosis
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Size:390 g
(6)制备Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (6) Preparation of Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin 在步骤(5)的Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin中,加入脱帽试剂(20%哌啶的DMF溶液),常温反应5分钟,抽干,重新加入脱帽剂常温反应20分钟,用异丙醇洗涤3次,DMF洗涤3次,重蒸过的DMF洗涤2次,抽干,加入Fmoc-Asp(otBu)-OH1.1g,DIEA0.4ml,TBTU0.56g,HOBT0.2g,DMF10ml常温反应1.5小时,用真空泵抽干,用异丙醇洗涤3次,DMF洗涤3次,重蒸过的DMF洗涤2次,抽干,获得Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin; In the Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin in step (5), add decapping reagent (20% piperidine in DMF solution), react at room temperature for 5 minutes, drain, and add decapping reagent at normal temperature React for 20 minutes, wash with isopropanol for 3 times, DMF for 3 times, DMF for redistilled for 2 times, drain, add Fmoc-Asp(otBu)-OH1.1g, DIEA0.4ml, TBTU0.56g, HOBT0 .2g, DMF10ml normal temperature reaction for 1.5 hours, drained with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with DMF evaporated, and dried to obtain Fmoc-Asp(otBu)-Asp(otBu )-Ala-Gly-Leu-Val-WangResin
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