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Genomic DNA (gDNA) from 4 tumors was pooled, and the sgRNA inserts were PCR-amplified, purified, and sequenced on the MiSeq platform (Illumina: RRID:SCR_016379)
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Size:20MG
Centrifuge (16,000 × g , 1 min) 1.2 ml of the residual yeast culture in a 100 mM EDTA solution and resuspend the pellet in 1 ml of ultrapure water by vortexing
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