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10 µL of methanol containing deuterated internal standard solution, a mixture of d4 PGF1α, d4 TXB2, d4 PGE2, d4 LTB4, d11 14,15 DiHETrE, d6 20 HETE, d4 9 HODE, d8 5 HETE, d11 11,12 EpETrE (Cayman Chemical, Ann Arbor, MI), was added to approximately 100 mg of liver tissue

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For IR-induced senescence, cells were exposed to 10Gy X- or Gamma rays, and analyzed 10 days later or otherwise indicated