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Furthermore, myeloid-derived suppressor cells (MDSCs), regulatory T cells (Tregs), and dysfunctional dendritic cells further impede CTL activity
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The T7 promoter sequence was included into the forward primer, and genomic fragments were PCR amplified from SW480 cDNA, sequence-verified, and used as templates for in vitro transcription with the T7 RiboMAX Express Large-Scale RNA Production System (Promega) per the manufacturer’s instructions
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